東京ノニ研究所 Dr. Noni
HPLC-UV quantification of two iridoids in Noni (Morinda
Rie Ikeda1,2, Mitsuhiro Wada1,2, Toshiaki
Nishigaki3, Shigeru Kawakami1,2, Naotaka Kuroda1,2
and Kenichiro Nakashima4
Department of Pharmacy, Nagasaki University, Japan 2
Graduate School of Biomedical Sciences, Nagasaki University,
3 Tokyo Noni Research Center, Japan 4 Faculty of
Pharmaceutical Sciences, Nagasaki International University, Japan
commonly known as “Noni” has a long history of wide use as a food in
Noni products have spread widely through the Internet primarily,
especially since the approval of noni fruit juice from Flench Polynesua
as a novel food by the Commission of the European Union in 2003.
However, scientific evidence in regard to quality control, safety and
usefulness of Noni products has not been completely established.
For their establishment, determination method of the ingredients of noni
Therefore the aim of this study is to develop a quantitative method with
HPLC-UV for two iridoids, deacetylasperulosidic acid (DAA) and
asperulosidic acid (AA), these are one of the active ingredients in Noni.
Noni products : Nine noni juice and 5 tea products commercially
available were used.
Sample pretreatment: Juice samples were diluted to 10 times with
methanol and centrifuged (1000g)
for 5 min.
The supernatant was filtered with membrane filter (0.45 μm), then
applied to HPLC analysis.
Tea samples were extracted with boiled water.
HPLC conditions : Separation of iridoids was performed on a ZIC-HILIC
column (250 × 4.6 nm, 5 μm) with a isocratic elution of acetonitrile/0.1%
formic acid (= 87/13).
Detection wavelength was 235 nm and injection volume was 20 μL.
【Results and Discussion】
iridoids with 26.6 min (DAA) and 11.0 min (AA) of retention time was
achieved by the
Also ascorbic acid (20.2 min) was separated.
These calibration curves showed good linearity
in the range of 0.5-100 (DAA), 0.1-20 (AA) and 7.5-150 μg/mL (ASA). Limit
of quantitation (at a signal-to-noise ratio of 10) were 0.200, 0.057 and
1.200 μg/mL, respectively.
Recovery was in the range of 95.7-109.2%, 103.0-108.0% and 27.3-93.0%.
Additionally the proposal method could be applied to determine the
iridoids in Noni products.
method could quantitate DAA, AA and ASA in Noni products with a simple
pretreatment and instruments.
Therefore this method might be useful for quality control of Noni