東京ノニ研究所公式サイトTokyo Noni Research Center

ホーム 上へ 2016年薬学会

東京ノニ研究所 Dr. Noni

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HPLC-UV quantification of two iridoids in Noni (Morinda citrifolia) products

 Yutaro Yamasaki1, Rie Ikeda1,2, Mitsuhiro Wada1,2, Toshiaki Nishigaki3, Shigeru Kawakami1,2, Naotaka Kuroda1,2 and Kenichiro Nakashima4

 1 Department of Pharmacy, Nagasaki University, Japan 2 Graduate School of Biomedical Sciences, Nagasaki University, Japan
3
Tokyo Noni Research Center, Japan 4 Faculty of Pharmaceutical Sciences, Nagasaki International University, Japan

E-mail: m-wada@nagasaki-u.ac.jp  

 Purpose
Morinda citrifolia commonly known as “Noni” has a long history of wide use as a food in Southeast Asia.
Noni products have spread widely through the Internet primarily, especially since the approval of noni fruit juice from Flench Polynesua as a novel food by the Commission of the European Union in 2003.
However, scientific evidence in regard to quality control, safety and usefulness of Noni products has not been completely established.
For their establishment, determination method of the ingredients of noni is required.
Therefore the aim of this study is to develop a quantitative method with HPLC-UV for two iridoids, deacetylasperulosidic acid (DAA) and asperulosidic acid (AA), these are one of the active ingredients in Noni.

Method
Noni products : Nine noni juice and 5 tea products commercially available were used.
Sample pretreatment: Juice samples were diluted to 10 times with methanol and centrifuged (1000g) for 5 min.
The supernatant was filtered with membrane filter (0.45 μm), then applied to HPLC analysis.
Tea samples were extracted with boiled water.
HPLC conditions : Separation of iridoids was performed on a ZIC-HILIC column (250 × 4.6 nm, 5 μm) with a isocratic elution of acetonitrile/0.1% formic acid (= 87/13).
Detection wavelength was 235 nm and injection volume was 20 μL.

Results and Discussion
Separation of iridoids with 26.6 min (DAA) and 11.0 min (AA) of retention time was achieved by the eluention conditions.
Also ascorbic acid (20.2 min) was separated.
These calibration curves
showed good linearity (r0.999) in the range of 0.5-100 (DAA), 0.1-20 (AA) and 7.5-150 μg/mL (ASA). Limit of quantitation (at a signal-to-noise ratio of 10) were 0.200, 0.057 and 1.200 μg/mL, respectively.
Recovery was in the range of 95.7-109.2%, 103.0-108.0% and 27.3-93.0%.
Additionally the proposal method could be applied to determine the iridoids in Noni products.

This method could quantitate DAA, AA and ASA in Noni products with a simple pretreatment and instruments.
Therefore this method might be useful for quality control of Noni products.